EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.84 | o-phenanthroline | inhibition of recombinant Ste24 CAAX proteolytic activity | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.84 | integral to membrane | Ste24 has a lumenal N-terminus and a cytosolic C-terminus indicativ of an odd number of transmembrane spans, hydropathy analysis suggests 7 membrane spans | Saccharomyces cerevisiae | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.84 | Co2+ | restores Ste24 CAAX proteolytic activity after 1,10-phenanthroline treatment, reactivation with 0.25 mM Co2+ is 25% of that seen with 0.25 mM Zn2+ | Saccharomyces cerevisiae | |
3.4.24.84 | Zn2+ | - |
Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.84 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.84 | recombinant His-tagged Ste24, nickel chelate chromatography | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.84 | YIIKGVFWDPA(farnesyl)CVIA + H2O | farnesylated 15-mer peptide containing the mature a-factor sequence and the native a-factor CAAX motif | Saccharomyces cerevisiae | YIIKGVFWDPA(farnesyl)C + Val-Ile-Ala | - |
? |